RNAi-mediated gene knockdown by microinjection in the model entomopathogenic nematode Heterorhabditis bacteriophora
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چکیده
منابع مشابه
RNAi mediated gene knockdown and transgenesis by microinjection in the necromenic Nematode Pristionchus pacificus.
Although it is increasingly affordable for emerging model organisms to obtain completely sequenced genomes, further in-depth gene function and expression analyses by RNA interference and stable transgenesis remain limited in many species due to the particular anatomy and molecular cellular biology of the organism. For example, outside of the crown group Caenorhabditis that includes Caenorhabdit...
متن کاملEntomopathogenic Nematode Genome Consortium: Sequencing and Annotation of Heterorhabditis bacteriophora Project Summary: This project aims to sequence the genome of the entomopathogenic nematode Heterorhabditis bacteriophora strain TTO1
Heterorhabditis bacteriophora Project Summary: This project aims to sequence the genome of the entomopathogenic nematode Heterorhabditis bacteriophora strain TTO1 as a representative of 45 other species that are used as biological control agents for insect pests worldwide. This nematode is closely related to the two already sequenced free-living nematodes, Caenorhabditis elegans and C. briggsae...
متن کاملComparative analysis of the expressed genome of the infective juvenile entomopathogenic nematode, Heterorhabditis bacteriophora.
We report the first cDNA-sequencing project of the entomopathogenic nematode, Heterorhabditis bacteriophora. A total of 1246 expressed sequence tags (ESTs) were generated by random sequencing of clones from a cDNA library of the infective juvenile stage. The ESTs were annotated resulting in 1072 useful ESTs that were categorized into functional categories according to Kyoto Encyclopedia of Gene...
متن کاملA Lover and a Fighter: The Genome Sequence of an Entomopathogenic Nematode Heterorhabditis bacteriophora
Heterorhabditis bacteriophora are entomopathogenic nematodes that have evolved a mutualism with Photorhabdus luminescens bacteria to function as highly virulent insect pathogens. The nematode provides a safe harbor for intestinal symbionts in soil and delivers the symbiotic bacteria into the insect blood. The symbiont provides virulence and toxins, metabolites essential for nematode reproductio...
متن کاملEnvironmental factors affecting sexual differentiation in the entomopathogenic nematode Heterorhabditis bacteriophora.
The present study was aimed at determining the influence of various environmental factors on sex differentiation (SD) in the entomopathogenic nematode Heterorhabditis bacteriophora HP88 strain, under in vivo and in vitro culture conditions. Injection of individual nematodes into last instars of Galleria mellonella resulted in development of a similar number of females and hermaphrodites (35-40%...
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ژورنال
عنوان ژورنال: Parasites & Vectors
سال: 2016
ISSN: 1756-3305
DOI: 10.1186/s13071-016-1442-4